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The results obtained matched the hypothesis which stated that 37c ? is the optimum temp for enzyme catalase to break hydrogen peroxide which is a toxic product into water & oxygen. According to our results in a table (2) and graph, the optimum temp was 37c? at which the rate of enzyme catalase activity was the highest as the highest volume of oxygen bubbles was produced 23. Below the optimum temp the kinetic energy of molecules decreases, therefore the collisions decrease and the enzyme would slow down and the rate of energy decreases as it’s shown in table (2) the volume of oxygen bubble decrease from 37c to 10c. This is shown in the graph, as the volume of oxygen bubbles cm3 decreased from 23.

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Above the optimum temp, the kinetic energy of molecules increases, therefore, the collisions between the active site and the substrate increase, and as a result, the enzyme would lose its 3D structure and active site and the enzyme would be denatured. at which the rate of enzyme activity is the highest.

  • The volume of hydrogen peroxide: 15cm3 of hydrogen peroxide was measured using a graduated cylinder for each trial at different temp.
  • If changed, then this would affect the rate of enzyme activity, therefore affecting the results. This is controlled by cutting all potato cubes with the same dimensions using a ruler & a cutter.
  • Size of potato cubes with dimensions 1cmx1cmx1.
  • If changed, whether decrease or increase, then the number of enzymes (active site) available would change, therefore affecting the rate of the reaction.
  • A number of potato cubes: 3 cubes of potatoes were used in each trial at each different temp.
  • Independent: Temperature (10c, 37c, 60c) using three different water baths each adjusted to a specific temp. Variables: Dependent: Height of oxygen bubbles (cm) using a ruler. )if increased to 60c? then the enzyme would be denatured and if decreased to 10c? (very low temp. It breaks down hydrogen peroxide (a waste product) into water and oxygen.Īs predicted, the enzyme catalase activity would be the highest at 37c? (Optimum temp.

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    Catalase is an enzyme, found basically in all living cells. The kinetic energy between molecules increases therefore collisions increase leading to the change in the tertiary structure of the enzyme and in this case active site is lost and the enzymes will be denatured so the reaction will slow down &stops. Order custom essay Effect of Temperature on Enzyme Catalase Activity in a PotatoĮnzymes are affected by several factors including PH, Substrate concentration Temperature & other factors.Įach enzyme has an optimum temperature at which its activity is the highest, below this optimum temp, the kinetic energy of molecules decreases, therefore the collisions between the active site of the enzyme and substrate decreases, as a result, the enzyme activity will decrease, so decreasing the rate of the reaction If the temp.















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